Grover, J. W., Bomhoff, M., Davey, S., Gregory, B. D., Mosher, R. A., & Lyons, E. (2017). CoGe LoadExp+: A web-based suite that integrates next-generation sequencing data analysis workflows and visualization. Plant Direct, 1(2).
BIO5 Collaborators
Eric H Lyons, Rebecca A Mosher
Mosher, R. A., & Baulcombe, D. C. (2008). Bacterial pathogens encode suppressors of RNA-mediated silencing.. Genome biology, 9(10), 237-.
PMID: 18947381;PMCID: PMC2760867;Abstract:
Plant pathogenic bacteria encounter host defenses mediated by a variety of small RNAs. Bacterial suppressors of silencing that inhibit multiple steps of plant microRNA biogenesis and function have recently been identified.
Huang, Y., Kendall, T., & Mosher, R. A. (2013). Pol IV-Dependent siRNA Production is Reduced in Brassica rapa. Biology, 2(4), 1210-1223.
Mosher, R. A., Tan, E. H., Shin, J., Fischer, R. L., Pikaard, C. S., & Baulcombe, D. C. (2011). An atypical epigenetic mechanism affects uniparental expression of Pol IV-dependent sirnas. PLoS ONE, 6(10).
PMID: 22003406;PMCID: PMC3189211;Abstract:
Background: Small RNAs generated by RNA polymerase IV (Pol IV) are the most abundant class of small RNAs in flowering plants. In Arabidopsis thaliana Pol IV-dependent short interfering (p4-si)RNAs are imprinted and accumulate specifically from maternal chromosomes in the developing seeds. Imprinted expression of protein-coding genes is controlled by differential DNA or histone methylation placed in gametes. To identify epigenetic factors required for maternal-specific expression of p4-siRNAs we analyzed the effect of a series of candidate mutations, including those required for genomic imprinting of protein-coding genes, on uniparental expression of a representative p4-siRNA locus. Results: Paternal alleles of imprinted genes are marked by DNA or histone methylation placed by DNA METHYLTRANSFERASE 1 or the Polycomb Repressive Complex 2. Here we demonstrate that repression of paternal p4-siRNA expression at locus 08002 is not controlled by either of these mechanisms. Similarly, loss of several chromatin modification enzymes, including a histone acetyltransferase, a histone methyltransferase, and two nucleosome remodeling proteins, does not affect maternal expression of locus 08002. Maternal alleles of imprinted genes are hypomethylated by DEMETER DNA glycosylase, yet expression of p4-siRNAs occurs irrespective of demethylation by DEMETER or related glycosylases. Conclusions: Differential DNA methylation and other chromatin modifications associated with epigenetic silencing are not required for maternal-specific expression of p4-siRNAs at locus 08002. These data indicate that there is an as yet unknown epigenetic mechanism causing maternal-specific p4-siRNA expression that is distinct from the well-characterized mechanisms associated with DNA methylation or the Polycomb Repressive Complex 2. © 2011 Mosher et al.
Gohlke, J., & Mosher, R. A. (2015). Exploiting mobile RNA silencing for crop improvement. American Journal of Botany (review article), 102(9), 1399-400. doi:10.3732/ajb.1500173
