Barton, J., Kanter, E. M., Walker, R. M., Marion, S. L., Brewer, M., Hoyer, P. B., & Barton, J. K. (0). Dual modality imaging of a novel rat model of ovarian carcinogenesis. Journal of biomedical optics, 11(4).
Ovarian cancer is the fifth leading cause of cancer death in women, in part because of the limited knowledge about early stage disease. We develop a novel rat model of ovarian cancer and perform a pilot study to examine the harvested ovaries with complementary optical imaging modalities. Rats are exposed to repeated daily dosing (20 days) with 4-vinylcyclohexene diepoxide (VCD) to cause early ovarian failure (model for postmenopause), and ovaries are directly exposed to 7,12-dimethylbenz(a)anthracene (DMBA) to cause abnormal ovarian proliferation and neoplasia. Harvested ovaries are examined with optical coherence tomography (OCT) and light-induced fluorescence (LIF) at one, three, and five months post-DMBA treatment. VCD causes complete ovarian follicle depletion within 8 months after onset of dosing. DMBA induces abnormal size, cysts, and neoplastic changes. OCT successfully visualizes normal and abnormal structures (e.g., cysts, bursa, follicular remnant degeneration) and the LIF spectra show statistically significant changes in the ratio of average emission intensity at 390:450 nm between VCD-treated ovaries and both normal cycling and neoplastic DMBA-treated ovaries. Overall, this pilot study demonstrates the feasibility of both the novel animal model for ovarian cancer and the ability of optical imaging techniques to visualize ovarian function and health.
Kostuk, R. K., Barton, J. K., Luo, Y., Castro, J. M., & Barbastathis, G. (2011). Volume Holographic Spectral-Spatial Imaging of Biological Tissue. TRIBUTE TO JOSEPH W. GOODMAN, 8122.
Barton, J. K., Amirsolaimani, B., Rice, P., Hatch, K., & Kieu, K. (2016). Three-photon imaging of ovarian cancer. PHOTONIC THERAPEUTICS AND DIAGNOSTICS XII, 9689.
Korde, V. R., Liebmann, E., & Barton, J. K. (2009). Design of a Handheld Optical Coherence Microscopy Endoscope. ENDOSCOPIC MICROSCOPY IV, 7172.
Howlett, I. D., Han, W., Rice, P., Barton, J. K., & Kostuk, R. K. (2017). Wavelength-coded volume holographic imaging endoscope for multidepth imaging. Journal of biomedical optics, 22(10), 1-4.
A wavelength-coded volume holographic imaging (WC-VHI) endoscope system capable of simultaneous multifocal imaging is presented. The system images light from two depths separated by 100 μm in a tissue sample by using axial chromatic dispersion of a gradient index probe in combination with two light-emitting diode sources and a multiplexed volume hologram to separate the images. This system is different from previous VHI systems in that it uses planar multiplexed gratings and does not require curved holographic gratings. This results in improved lateral imaging resolution from 228.1 to 322.5 lp/mm. This letter describes the design and fabrication of the WC-VHI endoscope and experimental images of hard and soft resolution targets and biological tissue samples to illustrate the performance properties.
