Watson, J. M., Rice, P. F., Marion, S. L., Brewer, M. A., Davis, J. R., Rodriguez, J. J., Utzinger, U., Hoyer, P. B., & Barton, J. K. (2012). Analysis of Second-Harmonic Generation Microscopy in a Mouse Model of Ovarian Carcinoma. Journal of Biomedical Optics, 17(7), 076002-1 to 076002-9.
Barton, J. K., Barton, J. K., Rice, P. F., Marion, S. L., Brewer, M. A., Davis, J. R., Rodriguez, J. J., Utzinger, U., Hoyer, P. B., & Barton, J. K. (2012). Analysis of second-harmonic-generation microscopy in a mouse model of ovarian carcinoma. Journal of biomedical optics, 17(7).
Second-harmonic-generation (SHG) imaging of mouse ovaries ex vivo was used to detect collagen structure changes accompanying ovarian cancer development. Dosing with 4-vinylcyclohexene diepoxide and 7,12-dimethylbenz[a]anthracene resulted in histologically confirmed cases of normal, benign abnormality, dysplasia, and carcinoma. Parameters for each SHG image were calculated using the Fourier transform matrix and gray-level co-occurrence matrix (GLCM). Cancer versus normal and cancer versus all other diagnoses showed the greatest separation using the parameters derived from power in the highest-frequency region and GLCM energy. Mixed effects models showed that these parameters were significantly different between cancer and normal (P0.008). Images were classified with a support vector machine, using 25% of the data for training and 75% for testing. Utilizing all images with signal greater than the noise level, cancer versus not-cancer specimens were classified with 81.2% sensitivity and 80.0% specificity, and cancer versus normal specimens were classified with 77.8% sensitivity and 79.3% specificity. Utilizing only images with greater than of 75% of the field of view containing signal improved sensitivity and specificity for cancer versus normal to 81.5% and 81.1%. These results suggest that using SHG to visualize collagen structure in ovaries could help with early cancer detection.
Black, J., Tate, T., Keenan, M., Swan, E., Utzinger, U., & Barton, J. (2015). A Six-Color Four-Laser Mobile Platform for Multi-Spectral Fluorescence Imaging Endoscopy. 2015 CONFERENCE ON LASERS AND ELECTRO-OPTICS (CLEO).
Winkler, A. M., Bonnema, G. T., & Barton, J. K. (2010). New Scheme for Polarimetric Glucose Sensing without Polarizers. OPTICAL DIAGNOSTICS AND SENSING X: TOWARD POINT-OF-CARE DIAGNOSTICS, 7572.
Keenan, M., Howard, C., Tate, T., McGuiness, I., Sauer-Budge, A., Black, J., Utzinger, U., & Barton, J. K. (2016). Design of an everting balloon to deploy a microendoscope to the fallopian tubes.. PHOTONIC THERAPEUTICS AND DIAGNOSTICS XII, 9689.